Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 48-7235-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-12/IL-23 p40 Monoclonal Antibody (eBioHP40 (HP40, HP-40)), eFluor™ 450, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The eBioHP40 antibody reacts with the p40 subunit of human IL-12 p70, and human IL-23. IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted primarily by activated monocytes, macrophages, and dendritic cells. IL-23 is a biologically-related, heterodimeric cytokine composed of disulfide-linked p40 and p19 subunits.
- Antibody clone number
- eBioHP40 (HP40, HP-40)
- Concentration
- 5 µL/Test
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of normal human peripheral blood cells cultured overnight with IFN gamma (Product # 14-8319-80), LPS and monensin (Product # 00-4505-51) with Anti-Human CD14 PE-Cyanine7 (Product # 25-0149-42) followed by fixation, permeabilization and subsequent intracellular staining with Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (left) or Anti-Human IL-12/IL-23 p40 eFluor® 450 (right). Total viable cells as determined by Fixable Viability Dye eFluor® 780 (Product # 65-0865-14) were used for analysis.