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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- 53-7129-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-IL-12/IL-23 p40 Monoclonal Antibody (C8.6), Alexa Fluor 488, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The C8.6 antibody reacts with the p40 subunit of human IL-12 p70 and human IL-23. IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and secreted primarily by activated monocytes, macrophages, and dendritic cells. IL-23 is a biologically-related, heterodimeric cytokine composed of disulfide-linked p40 and p19 subunits. Applications Tested: This C8.6 antibody has been pre-titrated and tested by intracellular staining of IFN gamma LPS actiavted human normal human peripheral blood cells. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- C8.6
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Antigen-specific activation and cytokine-facilitated expansion of naive, human CD8+ T cells.
Wölfl M, Greenberg PD
Nature protocols 2014 Apr;9(4):950-66
Nature protocols 2014 Apr;9(4):950-66
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Intracellular staining of unstimulated (left) or 2-hour IFN gamma and overnight LPS-stimulated (right) normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or Anti-Human IL-12 p40 Alexa Fluor® 488 (filled histogram). Cells in the monocyte gate were used for analysis.
- Conjugate
- Green dye
