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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-27620 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KARS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: H1299. Predicted reactivity: Mouse (94%), Rat (93%), Zebrafish (85%), Xenopus laevis (88%), Chicken (86%), Rhesus Monkey (96%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using KARS Polyclonal Antibody (Product # PA5-27620). Sample (30 µg of whole cell lysate). Lane A: H1299. 7.5% SDS PAGE. KARS Polyclonal Antibody (Product # PA5-27620) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Lysyl-tRNA synthetase in paraformaldehyde-fixed HeLa cells using a Lysyl-tRNA synthetase polyclonal antibody (Product # PA5-27620) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using Lysyl-tRNA synthetase (Product # PA5-27620) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- KARS Polyclonal Antibody detects Lysyl-tRNA synthetase protein at cytosol on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. KARS Polyclonal Antibody (Product # PA5-27620) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
