Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00008565-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00008565-M02, RRID:AB_566273
- Product name
- YARS monoclonal antibody (M02), clone 2F3
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant YARS.
- Antigen sequence
MGDAPSPEEKLHLITRNLQEVLGEEKLKEILKERE
LKIYWGTATTGKPHVAYFVPMSKIADFLKAGCEVT
ILFADLHAYLDNMKAPWELLELRVSYYENVIKAML
ESIGVPLEKLKFIKGTDYQLSKEYTLDVYRLSSVV
TQHDSKKAGAEVVKQVEHPLLSGLLYPGLQALDEE
YLKVDAQFGGIDQRKIFTFAEKYLPALGYSKRVHL
MNPMVPGLTGSKMSSSEEESKIDLLDRKEDVKKKL
KKAFCEPGNVENNGVLSFIKHVLFPLKSEFVILRD
EKWGGNKTYTAYVDLEKDFAAEVVHPGDLKNSVEV
ALNKLLDPIREKFNTPALKKLASAAYPDPSKQKPM
AKGPAKNSEPEEVIPSRLDIRVGKIITVEKHPDAD
SLYVEKIDVGEAEPRTVVSGLVQFVPKEELQDRLV
VVLCNLKPQKMRGVESQGMLLCASIEGINRQVEPL
DPPAGSAPGEHVFVKGYEKGQPDEELKPKKKVFEK
LQADFKISEECIAQWKQTNFMTKLGSISCKSLKGG
NIS- Isotype
- IgG
- Antibody clone number
- 2F3
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Impaired protein translation in Drosophila models for Charcot-Marie-Tooth neuropathy caused by mutant tRNA synthetases.
Dominant mutations in the tyrosyl-tRNA synthetase gene recapitulate in Drosophila features of human Charcot-Marie-Tooth neuropathy.
Niehues S, Bussmann J, Steffes G, Erdmann I, Köhrer C, Sun L, Wagner M, Schäfer K, Wang G, Koerdt SN, Stum M, Jaiswal S, RajBhandary UL, Thomas U, Aberle H, Burgess RW, Yang XL, Dieterich D, Storkebaum E
Nature communications 2015 Jul 3;6:7520
Nature communications 2015 Jul 3;6:7520
Dominant mutations in the tyrosyl-tRNA synthetase gene recapitulate in Drosophila features of human Charcot-Marie-Tooth neuropathy.
Storkebaum E, Leitão-Gonçalves R, Godenschwege T, Nangle L, Mejia M, Bosmans I, Ooms T, Jacobs A, Van Dijck P, Yang XL, Schimmel P, Norga K, Timmerman V, Callaerts P, Jordanova A
Proceedings of the National Academy of Sciences of the United States of America 2009 Jul 14;106(28):11782-7
Proceedings of the National Academy of Sciences of the United States of America 2009 Jul 14;106(28):11782-7
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- YARS monoclonal antibody (M02), clone 2F3. Western Blot analysis of YARS expression in NIH/3T3(Cat # L018V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged YARS is 0.1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
