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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-27434 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- YARS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: H1299. Predicted reactivity: Mouse (93%), Rat (90%), Chicken (82%), Bovine (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.25 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using YARS Polyclonal Antibody (Product # PA5-27434). Sample (30 µg whole cell lysate). A: H1299. 7.5% SDS PAGE. YARS Polyclonal Antibody (Product # PA5-27434) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Tyrosyl tRNA synthetase in methanol-fixed A431 cells using a Tyrosyl tRNA synthetase polyclonal antibody (Product # PA5-27434) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- YARS Polyclonal Antibody detects YARS protein at cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. YARS Polyclonal Antibody (Product # PA5-27434) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
