LS-C286692

antibody from LSBio

Targeting: POLR1G A34.5, ASE-1, CAST, CD3EAP, PAF49, RPA34

Immunocytochemistry (Enhanced validation)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C286692

Provider

LSBio

Product name

CD3EAP Antibody (aa404-454) LS-C286692

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at 2-8°C for up to one year.

Immunocytochemistry

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of Human PAF49/CAST by Immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-PAF49/CAST used at a dilution of 1:125. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte Plus 555 (A120-501E) used at a dilution of 1:100.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of Human PAF49/CAST by Immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-PAF49/CAST used at a dilution of 1:125. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody used at a dilution of 1:100.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human PAF49/CAST by Immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-PAF49/CAST used at a dilution of 1:125. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte Plus 555 (A120-501E) used at a dilution of 1:100.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human PAF49/CAST by Immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-PAF49/CAST used at a dilution of 1:125. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody used at a dilution of 1:100.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human PAF49/CAST by Immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti-PAF49/CAST used at a dilution of 1:125. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte Plus 555 (A120-501E) used at a dilution of 1:100.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of Human PAF49/CAST by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PAF49/CAST used at a dilution of 1:1,250. Epitope Retrieval Buffer-High pH (IHC-101J) was substituted for Epitope Retrieval Buffer-Reduced pH.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of Human PAF49/CAST by Immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-PAF49/CAST used at a dilution of 1:1,250. Epitope Retrieval Buffer-High pH (IHC-101J) was substituted for Epitope Retrieval Buffer-Reduced pH.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human PAF49/CAST by Immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PAF49/CAST used at a dilution of 1:1,250. Epitope Retrieval Buffer-High pH (IHC-101J) was substituted for Epitope Retrieval Buffer-Reduced pH.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection of Human PAF49/CAST by Immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-PAF49/CAST used at a dilution of 1:1,250. Epitope Retrieval Buffer-High pH (IHC-101J) was substituted for Epitope Retrieval Buffer-Reduced pH.