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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
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Validation data
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- Product number
- MA5-24628 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MKL2 Monoclonal Antibody (CL1546)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: QRPGPMELVE KNILPVDSSV KEAIIGVGKE DYPHTQGDFS FDEDSSDALS PDQPASQESQ GSAASPSEPK VSESPSPVTT NTPAQFASVS PTVPEFLKTP PTADQPPPRP AAPVLPTNTV SSAKPGPALV KQSHPKNP Highest antigen sequence identity to the following orthologs: Mouse - 89%, Rat - 54%. Binds to an epitope located within the peptide sequence FASVSPTVPEFLKTP as determined by overlapping synthetic peptides.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- CL1546
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MKL2 in Lane1: Marker (kDa), Lane 2: Human cell line RT-4. Samples were probed using a MKL2 Monoclonal Antibody (Product # MA5-24628).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of MKL2 in A549 cells using MKL2 Monoclonal Antibody (CL1546) (Product # MA5-24628), showing specific staining in the nucleus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of MKL2 in A549 cells using MKL2 Monoclonal Antibody (CL1546) (Product # MA5-24628) shows specific staining in nuclear speckles and the cytosol in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available).
