Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-C745319 - Provider product page
- Provider
- LSBio
- Product name
- CENPU / MLF1IP Antibody LS-C745319
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot using the affinity purified anti-MLF1IP antibody shows detection of endogenous MLF1IP protein (a tier of four modified protein bands indicated by the arrowheads) in lysates of Hela cells treated with control luciferase shRNA (lane 1), and detection of MLF1IP in Hela cells transfected with MLF1IP (lane 3). Lane 2: Hela cells treated with MLF1IP shRNA. The identity of the lower molecular weight bands is unknown. Primary antibody was used at 1:1,000.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunohistochemistry of rabbit anti-Pogz antibody. Tissue: prostate. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-Pogz at 5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Pogz as precipitated red signal with hematoxylin purple nuclear counterstain.