Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- PA1-28530 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Collagen I Polyclonal Antibody, Biotin
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- PA1-28530 detects Collagen I from bovine, human samples.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Rabbit
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot detection of Collagen I in 100 µg per lane of (lane 1) Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) and (lane 2) PPARg-transduced cell lysates. Protein staining using a Collagen I polyclonal antibody (Product # PA1-28530) at a dilution of 0.2-2 µg/10 mL followed by incubation overnight at 4°C. Secondary detection was performed using an HRP-conjugated rabbit secondary antibody at 1 µg/10 mL overnight at 4°C. Samples were blocked TBS with 5% Non-fat milk. Predicted/Observed size: 138.9 kDa for Collagen I.
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot of Rabbit anti-Collagen I Polyclonal Antibody Collagen I Polyclonal Antibody, Biotin (Product # PA1-28530). Lane 1: Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced). Lane 2: PPARg-transduced cell lysates. Load: 100 µg per lane. Protein staining shown below each blot depicts equal protein loading. Primary antibody: anti-Collagen I Polyclonal Antibody at 0.2–2 µg/10 mL for overnight at 4ºC. Secondary antibody: horseradish peroxidase-conjugated rabbit secondary antibody at 1 µg/10 mL for overnight at 4ºC. Block: TBS with 5% Non-fat milk. Predicted/Observed size: 138.9 kDa for Collagen I. Other band (s): none.
- Conjugate
- Biotin
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of Collagen I was performed in formalin fixed, paraffin embedded human skin at pH 6. Antigen retrieval was not required, the Collagen I Polyclonal Antibody, Biotin (Product # PA1-28530) was used at 10 µg/mL for 1 h at RT, and a peroxidase rabbit secondary antibody was used at 1:10,000 for 45 min at RT. Localization: Collagen Type I is secreted in the extracellular matrix. Staining: Collagen Type I as precipitated brown signal (A) with hematoxylin purple nuclear counterstain. With corresponding negative conrol (B).
- Conjugate
- Biotin
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of Collagen I was performed in primary adult human dermal fibroblast cells without stimulation. The primary antibody used was a Collagen I Polyclonal Antibody, Biotin (Product # PA1-28530) at 5 µg/mL for 45 min at 4°C. Secondary antibody: Rabbit Streptavidin, R-PE antibody at 1:500 for 15 min at RT.
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of Collagen I was performed in mouse lung cells without any stimulation. The primary antibody used was a Collagen I Polyclonal Antibody, Biotin (Product # PA1-28530) and PE-conjugated CD45 and PE-conjugated anti-collagen type I was used as a secondary antibody.
- Conjugate
- Biotin