- MA1-26771 - Invitrogen Antibodies COL1A2 antibody

Submitted references Effects and Mechanism of Particulate Matter on Tendon Healing Based on Integrated Analysis of DNA Methylation and RNA Sequencing Data in a Rat Model.

Lee SY, Lee MH, Jo SK, Yoo IH, Sarankhuu BE, Kim HJ, Kang YE, Lee SE, Kim TY, Park MH, Lee CS, Han SY, Moon JH, Jung JY, Hong GL, Yoo NJ, Yoon ES, Choi JK, Won HR, Son JW, Song JH
International journal of molecular sciences 2022 Jul 25;23(15)

Local accumulation of extracellular matrix regulates global morphogenetic patterning in the developing mammary gland.

Nerger BA, Jaslove JM, Elashal HE, Mao S, Košmrlj A, Link AJ, Nelson CM
Current biology : CB 2021 May 10;31(9):1903-1917.e6

An Aurora Kinase B-Based Mouse System to Efficiently Identify and Analyze Proliferating Cardiomyocytes.

Fu W, Liao Q, Li L, Shi Y, Zeng A, Zeng C, Wang WE
Frontiers in cell and developmental biology 2020;8:570252

Bioengineering the Oxygen-Deprived Tumor Microenvironment Within a Three-Dimensional Platform for Studying Tumor-Immune Interactions.

Bhattacharya S, Calar K, Evans C, Petrasko M, de la Puente P
Frontiers in bioengineering and biotechnology 2020;8:1040

Direct reprogramming of human smooth muscle and vascular endothelial cells reveals defects associated with aging and Hutchinson-Gilford progeria syndrome.

Bersini S, Schulte R, Huang L, Tsai H, Hetzer MW
eLife 2020 Sep 8;9

Nox4 Expression Is Not Required for OVX-Induced Osteoblast Senescence and Bone Loss in Mice.

Chen JR, Lazarenko OP, Zhao H, Wankhade UD, Pedersen K, Watt J, Ronis MJJ
JBMR plus 2020 Aug;4(8):e10376

Recombinant AAV-CEA Tumor Vaccine in Combination with an Immune Adjuvant Breaks Tolerance and Provides Protective Immunity.

Hensel JA, Khattar V, Ashton R, Ponnazhagan S
Molecular therapy oncolytics 2019 Mar 29;12:41-48

Human mesenchymal stem cell-derived extracellular vesicles/estrogen combined therapy safely ameliorates experimentally induced intrauterine adhesions in a female rat model.

Ebrahim N, Mostafa O, El Dosoky RE, Ahmed IA, Saad AS, Mostafa A, Sabry D, Ibrahim KA, Farid AS
Stem cell research & therapy 2018 Jun 28;9(1):175

Three-dimensional poly lactic-co-glycolic acid scaffold containing autologous platelet-rich plasma supports keloid fibroblast growth and contributes to keloid formation in a nude mouse model.

Chen C, Wang H, Zhu G, Sun Z, Xu X, Li F, Luo S
Journal of dermatological science 2018 Jan;89(1):67-76

Qualitative and quantitative assessment of collagen and elastin in annulus fibrosus of the physiologic and scoliotic intervertebral discs.

Kobielarz M, Szotek S, Głowacki M, Dawidowicz J, Pezowicz C
Journal of the mechanical behavior of biomedical materials 2016 Sep;62:45-56

Afa/Dr-expressing, diffusely adhering Escherichia coli strain C1845 triggers F1845 fimbria-dependent phosphatidylserine externalization on neutrophil-like differentiated PLB-985 cells through an apoptosis-independent mechanism.

Sémiramoth N, Gleizes A, Turbica I, Sandré C, Marin-Esteban V, Gorges R, Servin A, Chollet-Martin S
Infection and immunity 2010 Jul;78(7):2974-83

Immune-reconstituted influenza virosome containing CD40L gene enhances the immunological and protective activity of a carcinoembryonic antigen anticancer vaccine.

Cusi MG, Del Vecchio MT, Terrosi C, Savellini GG, Di Genova G, La Placa M, Fallarino F, Moser C, Cardone C, Giorgi G, Francini G, Correale P
Journal of immunology (Baltimore, Md. : 1950) 2005 Jun 1;174(11):7210-6

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Collagen-I was performed in pig tongue tissue using Collagen I Monoclonal Antibody (COL-1) (Product # MA1-26771) at a dilution of 1:4,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Collagen-I was performed in pig tongue tissue using Collagen I Monoclonal Antibody (COL-1) (Product # MA1-26771) at a dilution of 1:4,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Collagen-I was performed in human tongue tissue using Collagen I Monoclonal Antibody (COL-1) (Product # MA1-26771) at a dilution of 1:2,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Collagen I Monoclonal Antibody (COL-1) detects Collagen I protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rabbit tongue. Collagen I stained by Collagen I Monoclonal Antibody (COL-1) (Product # MA1-26771) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Serum bone turnover markers in three different treatment mouse groups. Standard ELISA methods for ( A ) APL, alkaline phosphatase; ( B ) TRAP5b, tartrate-resistant acid phosphatase 5b; ( C ) CTX-1, C-terminal telopeptide of type I collagen. ( D ) Western blots for col-1a (collagen 1), NFATc1, and cathepsin K. Total protein was isolated from vertebra L2 to L3; samples were five per group. Data are expressed as mean +- SD (sham group n = 9; OVX group n = 10; OVX + E2 n = 11). One-way ANOVA followed by Student-Newman-Keuls post hoc analysis for multiple pairwise comparisons was performed.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Increased senescence pathway in bone from ovariectomized (OVX) Nox4 -/- mice. ( A ) Proteins were isolated from L2 to L3 vertebral bone from sham and OVX 8-month-old mouse groups. Western blots show overexpression p53, p21, p16, NFATc1, and cathepsin K, but downregulation of Col-1a protein expression in the OVX group compared with the sham group. ( B ) Senescence-associated beta-galactosidase (SAbetaG) staining was performed on cryo-sectioned L4 vertebral bone. Images (magnification x4 and x40 from an epifluorescent microscope, model BH-2; Olympus, Tokyo, Japan) show one representative sample from each group. White arrows show SabetaG-positive blue-stained osteoblastic cells on bone surface. ( C ) Western blots show overexpression of p53, p21, p16, NFATc1, and cathepsin K, but downregulation of Col-1a protein expression in 11-month-old OVX group compared with the sham group (two samples from the sham group were lost during sample preparation). ( D ) SAbetaG staining was performed on cryo-sectioned L4 vertebral bone. Images (magnification x4 and x40 from epifluorescent microscope, model BH-2; Olympus) show one representative sample from each group of 11-month-old Nox4 -/- mice. White arrows show SabetaG-positive blue-stained osteoblastic cells on bone surface.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 4 Low-oxygen within 3D-O matrices increases the expression of main fibrous ECM proteins. (A) Representative fluorescent images exhibiting changes in expression of collagen I, collagen III, and fibronectin at day 4 for MDA-MB-231 cells grown within 3D-O physiological and 3D-O tumorous matrices; DAPI: Blue; ECMs: green). Scale bar = 100 mum. (B) ECM expression by BCa cells growing within 3D-O physiological and 3D-O tumorous matrices, quantified as MFI of ECM expression (i) and the number of cells (ii) . (C) Representative fluorescent images exhibiting changes in collagen I expression by MDA-MB-231 cells grown in 3D-O physiological and 3D-O tumorous matrices after treatment with PX-478 for 4 days at 5 muM concentration. Scale bar = 100 mum. ** p < 0.001, * p < 0.05, data were analyzed using unpaired two-tailed Student''s t -test.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 2 The proliferating ventricular cardiomyocytes were efficiently labeled in Tnnt2-Dre x Aurkb-rox-tdTomato neonates. (A) Tnnt2-Dre mice were used to cross with Aurkb-rox-tdTomato mice to label proliferating cardiomyocytes. (B) Immunostaining showed the coexpression of tdTomato with cTnT, PECAM, a-SMA, or Col1 on P1 Tnnt2-Dre x Aurkb-rox-tdTomato heart sections. Scale bar = 40 mum. (C) Immunostaining for tdTomato with Aurkb on P56 Tnnt2-Dre x Aurkb-rox-tdTomato heart sections. Scale bar = 20 mum. (D) Representative images of flow cytometry analysis (D1) and quantification of tdTomato +- cells (D2) and numbers in G1, S, G2/M phases (D3) from ventricles of P1 Tnnt2-Dre x Aurkb-rox-tdTomato mice. (D2) n = 6, * p < 0.05 vs. tdTomato - cTnT - ; # p < 0.05 vs. tdTomato + cTnT - ; & p < 0.05 vs. tdTomato - cTnT + . (D3) n = 5, * p < 0.05 vs. tdTomato - cTnT + . (E) Representative images (E1) and quantification (E2) of time-lapse imaging analysis showed cell proliferation in vitro of tdTomato + cardiomyocytes from the ventricles of P1 Tnnt2-Dre x Aurkb-rox-tdTomato mice. Scale bar = 20 mum.

MA1-26771

Antibody data