Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NBP2-22311 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal EPRS Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EPRS Antibody [NBP2-22311] - Whole cell lysate (50 ug) from 293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. NBP2-22311 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: EPRS Antibody [NBP2-22311] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22311 used for IP at 6 ug/mg lysate. EPRS was also immunoprecipitated by rabbit anti-EPRS antibodies NBP2-22309 and NBP2-22310. For blotting immunoprecipitated EPRS, NBP2-22311 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: EPRS Antibody [NBP2-22311] - Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti- EPRS used at a dilution of 1:1,000 (1ug/ml) Detection: DAB. Counterstain: Hematoxylin (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: EPRS Antibody [NBP2-22311] - FFPE serial sections of human breast carcinoma. used at a dilution of 1:1,000