- AHO1202 - Invitrogen Antibodies MAPK14 antibody

Submitted references Antitumor Activity of Chitosan-Coated Iron Oxide Nanocomposite Against Hepatocellular Carcinoma in Animal Models.

Badawy MMM, Abdel-Hamid GR, Mohamed HE
Biological trace element research 2023 Mar;201(3):1274-1285

Empagliflozin mitigates type 2 diabetes-associated peripheral neuropathy: a glucose-independent effect through AMPK signaling.

Abdelkader NF, Elbaset MA, Moustafa PE, Ibrahim SM
Archives of pharmacal research 2022 Jul;45(7):475-493

Gamma Radiation-Attenuated Toxoplasma gondii Provokes Apoptosis in Ehrlich Ascites Carcinoma-Bearing Mice Generating Long-Lasting Immunity.

Hafez EN, Moawed FSM, Abdel-Hamid GR, Elbakary NM
Technology in cancer research & treatment 2020 Jan-Dec;19:1533033820926593

Pristimerin protects against doxorubicin-induced cardiotoxicity and fibrosis through modulation of Nrf2 and MAPK/NF-kB signaling pathways.

El-Agamy DS, El-Harbi KM, Khoshhal S, Ahmed N, Elkablawy MA, Shaaban AA, Abo-Haded HM
Cancer management and research 2019;11:47-61

Growth-factor receptor-bound protein-2 (Grb2) signaling in B cells controls lymphoid follicle organization and germinal center reaction.

Jang IK, Cronshaw DG, Xie LK, Fang G, Zhang J, Oh H, Fu YX, Gu H, Zou Y
Proceedings of the National Academy of Sciences of the United States of America 2011 May 10;108(19):7926-31

The mitogen-activated protein kinases (MAPK) p38 and JNK are markers of tumor progression in breast carcinoma.

Davidson B, Konstantinovsky S, Kleinberg L, Nguyen MT, Bassarova A, Kvalheim G, Nesland JM, Reich R
Gynecologic oncology 2006 Sep;102(3):453-61

TNF-alpha promotes a stop signal that inhibits neutrophil polarization and migration via a p38 MAPK pathway.

Lokuta MA, Huttenlocher A
Journal of leukocyte biology 2005 Jul;78(1):210-9

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot AnalysisProteins from extracts of human Jurkat cells (lane 1) or mouse Rawcells (lane 2) were resolved by SDS-PAGE and transferred to PVDF.The membranes were incubated with this antibody at a concentrationof 1 µg/mL. After washing, the

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of p38 was performed by loading 50 ng of human, recombinant p38-alpha, beta, gamma and delta per well onto a Novex 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BOX ). Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product # 62288), and blocked with 5% milk in TBST for one hour at room temperature. Isoforms of p38 were detected using a p38 polyclonal antibody (Product # AHO1202, left panel) and a pan-p38 antibody (right panel) at a dilution of 1 µg/mL in blocking buffer overnight at 4C on a rocking platform, followed by a Goat anti-Rabbit IgG HRP-linked secondary antibody (Product # 31460) at a dilution of 1:10,000 for at least 30 minutes. Chemiluminescent detection was performed using SuperSignal Pico (Product # 34078) and the myECL™ Imager (Product # 62236)

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of p38 MAPK was done on 70% confluent log phase HeLa cells treated with UV-302nm for 60 minutes and cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with of p38 MAPK Rabbit Polyclonal Antibody (Product # AHO1202) at 1:250 dilution in0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing nuclear and cytoplasmic localization. Panel e is untreated cells with no signal. Panel f is a no primary antibody control. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of p38-alpha (green) in HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 10 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a p38 polyclonal antibody (Product # AHO1202) at a dilution of 10 µg/mL in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor 488 conjugate (Product # A27034) at a dilution of 1:1000 for 1 hour at room temperature (green). Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of p38 MAPK showing staining in the cytoplasm and nucleus of paraffin-embedded rat heart tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a p38 MAPK polyclonal antibody (Product # AHO1202) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Effect of EMPA on NF-kappaB p65, p38 MAPK, ERK1/2, RECK, and miR-21 expressions in the sciatic nerves in STZ-induced DPN in rats. Panels represent protein expression of ( a ) p-NF-kappaB p65/total NF-kappaB p65, ( b ) p-p38 MAPK/total p38 MAPK, ( c ) p-ERK1/2/total ERK1/2, ( d ) RECK, and ( e ) corresponding western blotting bands along with relative expression of (f) miR-21. Results are displayed as mean +- SD (n = 3-4). (*) vs CONT, (@) vs STZ, (#) vs EMPA; P < 0.05. CONT: control; DPN: diabetic peripheral neuropathy; DORS: dorsomorphin; EMPA: empagliflozin; ERK: extracellular signal-regulated kinases; MAPK: mitogen-activated protein kinase; miR: micro-RNA; NF-kappaB: nuclear factor kappa-B; RECK: reversion-inducing cysteine-rich protein with Kazal motifs; STZ: streptozotocin

AHO1202

Antibody data