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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA504561 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA504561, RRID:AB_11128591
- Product name
- C18orf8 mouse monoclonal antibody, clone OTI4E4 (formerly 4E4)
- Antibody type
- Monoclonal
- Description
- C18orf8 mouse monoclonal antibody, clone OTI4E4 (formerly 4E4)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- RMC1
- Isotype
- IgG
- Antibody clone number
- OTI4E4
- Vial size
- 100 µl
- Concentration
- NULL
Submitted references Systematic Analysis of Human Cells Lacking ATG8 Proteins Uncovers Roles for GABARAPs and the CCZ1/MON1 Regulator C18orf8/RMC1 in Macroautophagic and Selective Autophagic Flux.
Vaites LP, Paulo JA, Huttlin EL, Harper JW
Molecular and cellular biology 2018 Jan 1;38(1)
Molecular and cellular biology 2018 Jan 1;38(1)
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C18orf8 (RC203449, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C18orf8.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC203449 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-C18orf8 antibody(TA504561), and then analyzed by flow cytometry.
- Validation comment
- FC
