H00011044-M01
antibody from Novus Biologicals
Targeting: TENT4A
LAK-1, PAPD7, POLK, POLS, TRF4, TRF4-1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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Validation data
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- Product number
- H00011044-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00011044-M01, RRID:AB_538317
- Product name
- Mouse Monoclonal DNA polymerase sigma Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. POLS - polymerase (DNA directed) sigma
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DNA polymerase sigma Antibody (2F8) [H00011044-M01] - Analysis of POLS expression in transfected 293T cell line by POLS monoclonal antibody (M01), clone 2F8. Lane 1: POLS transfected lysate (59.9 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DNA polymerase sigma Antibody (2F8) [H00011044-M01] - Analysis of POLS over-expressed 293 cell line, cotransfected with POLS Validated Chimera RNAi ( Cat # H00011044-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with POLS monoclonal antibody (M01), clone 2F8 (Cat # H00011044-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: DNA polymerase sigma Antibody (2F8) [H00011044-M01] - Detection limit for recombinant GST tagged POLS is approximately 0.3ng/ml as a capture antibody.