NBP2-13729

antibody from Novus Biologicals

Targeting: TENT4A LAK-1, PAPD7, POLK, POLS, TRF4, TRF4-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-13729

Provider

Novus Biologicals

Product name

Rabbit Polyclonal DNA polymerase sigma Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human, mouse DNA polymerase sigma antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: DNA polymerase sigma Antibody [NBP2-13729] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: DNA polymerase sigma Antibody [NBP2-13729] - Analysis in human cell line HeLa.

Immunocytochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: DNA polymerase sigma Antibody [NBP2-13729] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DNA polymerase sigma Antibody [NBP2-13729] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DNA polymerase sigma Antibody [NBP2-13729] - Staining of human colon, liver, lymph node and testis using Anti-PAPD7 antibody NBP2-13729 (A) shows similar protein distribution across tissues to independent antibody NBP2-13728 (B).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DNA polymerase sigma Antibody [NBP2-13729] - Staining of human lymph node shows strong nuclear positivity in non-germinal center cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DNA polymerase sigma Antibody [NBP2-13729] - Staining of human colon shows strong nuclear positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DNA polymerase sigma Antibody [NBP2-13729] - Staining of human liver shows weak nuclear positivity in hepatocytes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: DNA polymerase sigma Antibody [NBP2-13729] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.