GTX101741

antibody from GeneTex

Targeting: ATP5F1A ATP5A, ATP5A1, ATP5AL2, ATPM, hATP1, OMR, ORM

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX101741

Provider

GeneTex

Proper citation

GeneTex Cat#GTX101741, RRID:AB_10618791

Product name

ATP5A1 antibody [C2C3], C-term

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

The orphan nuclear receptor NR4A1 (Nur77) regulates oxidative and endoplasmic reticulum stress in pancreatic cancer cells.

Lee SO, Jin UH, Kang JH, Kim SB, Guthrie AS, Sreevalsan S, Lee JS, Safe S
Molecular cancer research : MCR 2014 Apr;12(4):527-538

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: Hep G2 (GTX27900) 7.5% SDS PAGE GTX101741 diluted at 1:500

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX101741 diluted at 1:1000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ATP5A1 antibody detects ATP5A1 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody (GTX101741) diluted by 1:2000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various tissue extracts (50 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody [C2C3], C-term (GTX101741) diluted at 1:1000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody (GTX101741) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ATP5A1 protein stained by ATP5A1 antibody [C2C3], C-term (GTX101741) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. ATP5A1 antibody [C2C3], C-term (GTX101741) dilution: 1:200.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein at cytoplasm on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. ATP5A1 antibody [C2C3], C-term (GTX101741) dilution: 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein at membrane on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. ATP5A1 antibody [C2C3], C-term (GTX101741) dilution: 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Green: ATP5A1 protein stained by ATP5A1 antibody [C2C3], C-term (GTX101741) diluted at 1:250.Red: Protein kinase C alpha staining.Blue: Fluoroshield with DAPI (GTX30920).