MAB70051
antibody from R&D Systems
Targeting: VSIR
B7-H5, B7H5, C10orf54, Dies1, GI24, PD-1H, SISP1, VISTA
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- MAB70051 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse VISTA/B7-H5/PD-1H Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse VISTA/B7-H5/PD-1H in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human VISTA/B7-H5/PD-1H is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q9D659
- Isotype
- IgG
- Antibody clone number
- 742002
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse VISTA/B7-H5/PD-1H by Western Blot. Western blot shows lysates of mouse placenta tissue, mouse spleen tissue, mouse lung tissue, and mouse platelets. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse VISTA/B7-H5/ PD-1H Monoclonal Antibody (Catalog # MAB70051) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for VISTA/B7-H5/PD-1H at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- VISTA/B7-H5/PD-1H in D3 Mouse Stem Cells. VISTA/B7-H5/PD-1H was detected in immersion fixed D3 mouse embryonic stem cell line using Rat Anti-Mouse VISTA/B7-H5/PD-1H Monoclonal Antibody (Catalog # MAB70051) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.