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LearnFAB7005A
antibody from R&D Systems
Targeting: VSIR
B7-H5, B7H5, C10orf54, Dies1, GI24, PD-1H, SISP1, VISTA
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [2]
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- Product number
- FAB7005A - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse VISTA/B7-H5/PD-1H APC-conjugated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse VISTA/B7-H5/PD-1H in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human VISTA/B7-H5/PD-1H is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Conjugate
- Red dye
- Antigen sequence
Q9D659- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of VISTA/B7-H5/PD-1H in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Sheep Anti-Mouse VISTA/B7-H5/PD-1H APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB7005A) or (B) Normal Sheep IgG Allophycocyanin Control (Catalog # IC016A). View our protocol for Staining Membrane-associated Proteins.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of VISTA/B7-H5/PD-1H in HEK293 Human Cell Line Transfected with Mouse VISTA and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse VISTA or (B) irrelevant transfectants and eGFP was stained with Sheep Anti-Mouse VISTA/B7-H5/PD-1H APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB7005A). Quadrant markers were set based on control antibody staining (Catalog # IC016A). View our protocol for Staining Membrane-associated Proteins.
