PA5-52493

antibody from Invitrogen Antibodies

Targeting: VSIR B7-H5, B7H5, C10orf54, Dies1, GI24, PD-1H, SISP1, VISTA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-52493

Provider

Invitrogen Antibodies

Product name

VISTA Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: LTFQDLHLHH GGHQAANTSH DLAQRHGLES ASDHHGNFSI TMRNLTLLDS GLYCCLVVEI RHHHSEHRVH GAMELQVQTG KDAPSNCVVY PSSS

Concentration

0.1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of C10orf54 in control (vector only transfected HEK293T lysate) and C10orf54 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a C10orf54 Polyclonal Antibody (Product # PA5-52493).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of C10orf54 in human cell lines A-431 and HEK293 using a C10orf54 Polyclonal Antibody (Product # PA5-52493). Corresponding VSIR RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of C10orf54 in human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a C10orf54 Polyclonal Antibody (Product # PA5-52493).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of C10orf54 in human tonsil using a C10orf54 Polyclonal Antibody (Product # PA5-52493) shows moderate to strong cytoplasmic positivity in a subset of lymphoid cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of C10orf54 in human liver using a C10orf54 Polyclonal Antibody (Product # PA5-52493) shows strong cytoplasmic positivity in leukocytes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of C10orf54 in human fallopian tube using a C10orf54 Polyclonal Antibody (Product # PA5-52493) shows strong cytoplasmic positivity in leukocytes.