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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [2]
- Other assay [1]
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- Product number
- MA5-24690 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- H4K20me3 Recombinant Rabbit Monoclonal Antibody (RM208)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibodyreacts to Histone H4 trimethylated at Lysine 20 (K20me3). No cross reactivity with non-modified Lysine 20, monomethylated Lysine 20 (K20me1), or other methylations in histone H4. Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- RM208
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot of acid extracts of HeLa cells (1) and recombinant Histone H4 (2), using Tri-Methyl-Histone H4 (Lys20) Antibody (Product # MA5-24690) at 1 mg/mL, showed a band of Histone H4 trimethylated at Lysine 20 (K20me3) in HeLa cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Tri-Methyl-Histone H4 (Lys20) Antibody (Product # MA5-24690) specifically reacts to Histone H4 trimethylated at Lysine 20 (K20me3). Monomethylated Lysine 20 (K20me1), or other methylations in Histone H4.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Tri-Methyl-Histone H4 (Lys20) Antibody (Product # MA5-24690) specifically reacts to Histone H4 trimethylated at Lysine 20 (K20me3). Monomethylated Lysine 20 (K20me1), or other methylations in Histone H4.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Tri-Methyl-Histone H4 (Lys20) Antibody (Product # MA5-24690) specifically reacts to Histone H4 trimethylated at Lysine 20 (K20me3). Monomethylated Lysine 20 (K20me1), or other methylations in Histone H4.
