Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [1]
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Validation data
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- Product number
- NB21-2090 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Histone H4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 mg
- Concentration
- 0.58 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references H4K20 methylation regulates quiescence and chromatin compaction.
Evertts AG, Manning AL, Wang X, Dyson NJ, Garcia BA, Coller HA
Molecular biology of the cell 2013 Oct;24(19):3025-37
Molecular biology of the cell 2013 Oct;24(19):3025-37
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NB21-2090] - Analysis of Histone H4 K20me3 in (A) HeLa histone lysate (B) NIH/3T3 histone lysate, and (C) C.elegans lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Histone H4 [Trimethyl Lys20] Antibody [NB21-2090] - Lane view shows a specific band for Histone H4 in 0.5 mg/ml of Hela Nuclear lysate. This experiment was performed under reducing A246 conditions using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Dot Blot: Histone H4 [Trimethyl Lys20] Antibody [NB21-2090] - Analysis of Histone H4 K20me3 in picomoles of peptide.