bs-4707R

antibody from Bioss

Targeting: H4-16 HIST4H4, MGC24116

Immunocytochemistry

Antibody data

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Validation data

Product number: bs-4707R - Provider product page
Provider: Bioss
Product name: Acetyl Histone H4(K17) Antibody
Antibody type: Polyclonal
Description: KLH conjugated synthetic peptide derived from human Acetyl-Histone H4(K17)
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 100ul
Concentration: 1ug/ul
Storage: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.

H4-16 protein structure - bs-4707R shown in red.

Supportive validation

Submitted by: Bioss (provider)
Main image
Experimental details: Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Acetyl-Histone H4(K16) Polyclonal Antibody, Unconjugated (bs-4707R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Sample type: Rat
Other comments: Brain

Supportive validation

Submitted by: Bioss (provider)
Main image
Experimental details: Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti Acetyl-Histone H4(K16) Polyclonal Antibody, Unconjugated (bs-4707R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Sample type: Mouse
Other comments: Intestine

Supportive validation

Submitted by: Bioss (provider)
Main image
Experimental details: Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti Acetyl-Histone H4(K16) Polyclonal Antibody, Unconjugated (bs-4707R) at 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
Sample type: Mouse
Other comments: Intestine

Supportive validation

Submitted by: Bioss (provider)
Main image
Experimental details: Image kindly submitted by Piotr Mamczur as part of the free sample program. Immunofluorescent localization of acetylated histone H4 in mouse squamous cell cancer (KLN-205 cell line) with BS-4707R (1:20) antibody and FITC-labeled secondary antibodies (1:2000). The nuclei were stained with DAPI.
Sample type: Mouse