NBP2-42863
antibody from Novus Biologicals
Targeting: H4-16
HIST4H4, MGC24116
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
- Chromatin Immunoprecipitation [1]
- Other assay [1]
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Validation data
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- Product number
- NBP2-42863 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Histone H4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Xenopus
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of: A. 30 ug HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr) B. 30 ug HeLa whole cell lysate/extract (0.4 uM Trichostatin A treatment for 18 hr) 15 % SDS-PAGE Histone H4 (acetyl Lys8).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of HeLa whole cell lysate/extract: A. 30 ug whole cell lysate/extract of Histone H4 (acetyl K9) protein expressing HeLa cells (0.4uM TSA treatment for 18 hrs) B. Control with 2.5 ug of pre-immune rabbit IgG C. Immunoprecipitation of Histone H4 (acetyl K9) protein by 2.5 ug of Histone H4 (acetyl K9) antibody . 12% SDS-PAGE The immunoprecipitated Histone H4 (acetyl K9) protein was detected by Histone H4 (acetyl K9). EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of mouse duodenum. Histone H4K8ac (acetyl Lys8) antibody dilution: 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of mouse kidney. Histone H4K8ac (acetyl Lys8) antibody dilution: 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of rat hind brain. Histone H4K8ac (acetyl Lys8) antibody dilution: 1:500.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Cross-linked ChIP was performed with HeLa chromatin extract treated with Trichostatin A (0.4 uM for 18 h) and 5 ug of either control rabbit IgG or anti-Histone H4K8ac (acetyl Lys8) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or TEXT12.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Dot Blot: Histone H4 [ac Lys8] Antibody [NBP2-42863] - Analysis of anti-Histone H4 (acetyl Lys9) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H4 (acetyl Lys9) antibody 1:5000 dilution. A: Peptide samples of H4K8 un B: Peptide samples of H4K8ac C: Peptide samples of H4K16un D: Peptide samples of H4K16ac.