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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- NBP2-42862 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Histone H4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Xenopus
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of A. 30 ug HeLa whole cell lysate/extract B. 30 ug HeLa nuclear lysate/extract 15 % SDS-PAGE Histone H4 (tri methyl Lys20) antibody dilution: 1:2000
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of mouse duodenum. Histone H4K20me3 (trimethyl Lys20) antibody dilution: 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of rat fore brain. Histone H4K20me3 (trimethyl Lys20) antibody dilution: 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of mouse muscle. Histone H4K20me3 (trimethyl Lys20) antibody dilution: 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of mouse liver. Histone H4K20me3 (trimethyl Lys20) antibody dilution: 1:500.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Dot Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-42862] - Analysis of anti-Histone H4 (tri methyl Lys20) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H4 (tri methyl Lys20) antibody 1:5000 dilution. A: Peptide samples of H4K20 un B: Peptide samples of H4K20me1 C: Peptide samples of H4K20me2 D: Peptide samples of H4K20me3.
