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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- R20222 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Recombinant phospho-Histone H4 Antibody (Ser1)
- Antibody type
- Recombinant
- Description
- Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- RM194
- Vial size
- 25 µg, 100 µg
- Concentration
- 1 mg/ml in PBS with 50% Glycerol, 1% BSA and 0.09% sodium azide
- Storage
- Store the recombinant phospho-Histone H4 antibody at -20°C.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot of (1) acid extracts of HeLa cells and (2) recombinant Histone H4 using the recombinant phospho-Histone H4 antibody at 0.5 ug/ml showed a band of Histone H4 phosphorylated at Serine 1 in HeLa cells.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC/IF of HeLa cells using recombinant phospho-Histone H4 antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- The recombinant phospho-Histone H4 antibody specifically reacts to Histone H4 phosphorylated at Ser1 (H4S1p). The reactivity is not affected by neighboring Arg3 modifications (H4S1pR3me1 and H4S1pR3me2). No cross reactivity with Histone H2A phosphorylated at Ser1 (H2AS1p).
