Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- R20225 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Recombinant H4K8ac Antibody / Acetyl Histone H4 Lysine 8
- Antibody type
- Recombinant
- Description
- Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- RM201
- Vial size
- 25 µg, 100 µg
- Concentration
- 1 mg/ml in PBS with 50% Glycerol, 1% BSA and 0.09% sodium azide
- Storage
- Store the recombinant H4K8ac antibody at -20°C.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot test of (1) acid extracts of HeLa cells treated with sodium butyrate, (2) acid extracts of HeLa cells untreated, and (3) recombinant Histone H4 using recombinant H4K8ac antibody at 0.5 ug/ml showed a band of Histone H4 acetylated at Lysine 8 in HeLa cells.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC/IF staining of HeLa cells treated with sodium butyrate using recombinant H4K8ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- This recombinant H4K8ac antibody specifically reacts to Histone H4 acetylated at Lysine 8 (K8ac). No cross reactivity with unmodified Lysine 8 (K8 ctrl) or other acetylated Lysines.