Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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Validation data
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- Product number
- R20238 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Recombinant H4K20me3 Antibody / Trimethyl Histone H4 Lysine 20
- Antibody type
- Recombinant
- Description
- Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- RM208
- Vial size
- 25 µg, 100 µg
- Concentration
- 1 mg/ml in PBS with 50% Glycerol, 1% BSA and 0.09% sodium azide
- Storage
- Store the recombinant H4K20me3 antibody at -20°C.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot test of (1) acid extracts of HeLa cells and (2) recombinant Histone H4, using recombinant H4K20me3 antibody at 1 ug/ml, showed a band of Histone H4 trimethylated at Lysine 20 in HeLa cells.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- This recombinant H4K20me3 antibody specifically reacts to Histone H4 trimethylated at Lysine 20 (K20me3). May slightly cross react to dimethylated Lysine 20 (K20me2) at higher concentrations. No cross reactivity with non-modified or monomethylated Lysine 20 (K20me1), or other H4 methylations.