Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- ELISA [1]
- Other assay [2]
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- Product number
- TA347218 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Polyclonal H4 pan Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal H4 pan Antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- HIST4H4
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 50 µg
- Concentration
- 1.15 ?g/?l
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Determination of the titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H4pan, crude serum and flow through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:11,250.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ChIP assays using HeLa cells: ChIP kit on sheared chromatin from 10,000 cells using the SX-8G IP-Star automated system. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for the ACTB promoter and for the second exon of the MB gene. Image shows the recovery, expressed as a % of input (the relative amount of IP'd DNA compared to input DNA after qPCR analysis).
- Validation comment
- Assay
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- A Dot Blot analysis was performed to test the cross reactivity of the antibody against H4pan with the peptide used for immunization of the rabbit and other peptides containing unmodified sequences of different histones. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:10,000. Image shows a high specificity of the antibody for the specific peptide.
- Validation comment
- DB