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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Other assay [1]
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- Product number
- NBP2-61552 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Monoclonal Histone H4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. This antibody reacts to Histone H4 trimethylated at Lysine 20 (K20me3). It may slightly cross react to dimethylated Lysine 20 (K20me2) at a higher concentration. No cross reactivity with non-modified Lysine 20, monomethylated Lysine 20 (K20me1), or other methylations in histone H4.
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Histone H4 [Trimethyl Lys20] Antibody (208) [NBP2-61552] - (1) acid extracts of HeLa cells; (2) recombinant Histone H4, at 1 ug/mL, showed a band of Histone H4 trimethylated at Lysine 20 in HeLa cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Histone H4 [Methyl Lys20] Antibody (RM208) [NBP2-61552] - Western Blot of acid extracts of HeLa cells (1) and recombinant Histone H4 (2), using NBP2-61552, showed a band of Histone H4 trimethylated at Lysine 20 (K20me3) in HeLa cells.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Histone H4 [Trimethyl Lys20] Antibody (208) [NBP2-61552] - Specifically reacts to Histone H4 trimethylated at Lysine 20 (K20me3). May slightly cross react to dimethylated Lysine 20 (K20me2) at higher concentrations. No cross reactivity with non-modified Lysine 20, monomethylated Lysine 20 (K20me1), or other methylations in histone H4.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Multiplex Immunoassay: Histone H4 [Methyl Lys20] Antibody (RM208) [NBP2-61552] - NBP2-61552 specifically reacts to Histone H4 trimethylated at Lysine 20 (K20me3). May slightly cross react to dimethylated Lysine 20 (K20me2) when used at higher concentrations. No cross reactivity with non-modified Lysine 20, monomethylated Lysine 20 (K20me1), or other methylations in histone H4.
