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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Other assay [1]
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- Product number
- NBP2-61554 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Monoclonal Histone H4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. This antibody reacts to Histone H4 acetylated at Lysine 12 (K12ac). No cross reactivity with other acetylated Lysines in Histone H4.
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Histone H4 [ac Lys12] Antibody (202) [NBP2-61554] - Acid extracts from: (1) HeLa cells treated with sodium butyrate; (2) HeLa cells untreated, at 0.5 ug/mL, showed a band of Histone H4 acetylated at Lysine 12 in HeLa cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Histone H4 [ac Lys12] Antibody (RM202) [NBP2-61554] - Western Blot of acid extracts from: (1) HeLa cells treated with sodium butyrate; (2) HeLa cells untreated. Using NBP2-61554, showed a band of Histone H4 acetylated at Lysine 12 in HeLa cells.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Histone H4 [ac Lys12] Antibody (202) [NBP2-61554] - Specifically reacts to Histone H4 acetylated at Lysine 12 (K12ac). No cross reactivity with unmodified Lysine 16 (K16 ctrl), acetylated Lysine 5 (K5ac), Lysine 8 (K8ac), Lysine 16 (K16ac), Lysine 20 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Multiplex Immunoassay: Histone H4 [ac Lys12] Antibody (RM202) [NBP2-61554] - NBP2-61554 specifically reacts to Histone H4 acetylated at Lysine 12 (K12ac). No cross reactivity with unmodified Lysine 12 (K126 ctrl), acetylated Lysine 5 (K5ac), Lysine 8 (K8ac), Lysine 16 (K16ac), Lysine 20 (K20ac), Lysine 31 (K31ac), or Lysine 91 (K91) in Histone H4.
