39167

antibody from Active Motif
Targeting: H4-16 HIST4H4, MGC24116
Provider product page for 39167
Western blot
Chromatin Immunoprecipitation

Antibody data

Product number
39167 - Provider product page
Provider
Active Motif
Proper citation
Active Motif Cat#39167, RRID:AB_2636968
Product name
Histone H4K16ac antibody (pAb)
Antibody type
Polyclonal
Description
This Histone H4 acetyl Lys16 antibody (H4K16ac) has been validated for use in chromatin IP (3 - 10 µl per ChIP), ChIP-Seq & ChIP-chip (5 µl each) and Western blotting (1:1,000 - 1:5,000 dilution). Specificity for acetyl-lysine 16 was confirmed by dot blot and peptide inhibition with non-modified histone H4 peptide or peptides acetylated at lysines 5, 8 or 12. Because this modification is conserved from yeast to human, a wide range of reactivity is expected. Overnight incubation with this antibody is recommended for Western blotting. Due to cytoplasmic staining, this product is not recommended for IF.The modENCODE and NIH Roadmap Epigenomics Mapping Consortiums have implemented rigorous standardization criteria for all assays and reagents to be used. As part of this initiative, antibody specificity testing and the ability of the antibodies to work in ChIP-Seq were assessed in a large-scale study. This Histone H4 acetyl Lys16 antibody was validated for ChIP-Seq in the study (see References).
Reactivity
Human, Drosophila, Zebrafish
Host
Rabbit
Vial size
200 µl
Storage
Antibodies in solution can be stored at -20C for 2 years. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
H4-16 protein structure - 39167 shown in red.