Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
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Validation data
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- Product number
- PA5-17190 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDHA1/PDHA2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 56 µg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Pyruvate Dehydrogenase in extracts from HepG2 and 293 cells using Pyruvate Dehydrogenase polyclonal antibody (Product # PA5-17190).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of PDH was achieved by transfecting HEK-293 cells with PDH specific siRNAs (Silencer® select Product # s10244). Western blot analysis (Fig. a) was performed using whole cell extracts from PDH knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with PDHA1/PDHA2 Polyclonal Antibody (Product # PA5-17190, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to PDH.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-PDHA1/PDHA2 Polyclonal Antibody (Product # PA5-17190) and a band at ~43 kDa corresponding to PDH was observed in all the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HEK 293 (Lane 2), MDA-MB-231 (Lane 3), HL-60 (Lane 4), SK-OV-3 (Lane 5), PC-3 (Lane 6), PANC-1 (Lane 7), Mouse Liver (Lane 8), Rat Liver (Lane 9), Mouse Brown Adipose (Lane 10), Mouse Brain (Lane 11) were electrophoresed using Novex® NuPAGE® 4-12% % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).