Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-30837 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Nectin 4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, Jurkat, Raji. Predicted reactivity: Mouse (92%), Rat (94%), Dog (94%), Bovine (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.81 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PVRL4 using 30 µg of Raji lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PVRL4 polyclonal antibody (Product # PA5-30837) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Nectin 4 Polyclonal Antibody (Product # PA5-30837). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Nectin 4 Polyclonal Antibody (Product # PA5-30837) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using PVRL4 (Product # PA5-30837) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.