MA1-81209

antibody from Invitrogen Antibodies

Targeting: FCGR2B CD32, CD32B, FCG2, FCGR2

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-81209

Provider

Invitrogen Antibodies

Product name

CD32 Monoclonal Antibody (AT10)

Antibody type

Monoclonal

Antigen

Other

Description

The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. A suggested positive control for immunohistochemical applications is lymph node.

Antibody clone number

AT10

Concentration

1 mg/mL

References

Submitted references

Case study interpretation: Report from the ICCS Annual Meeting, Seattle, 2014.

Fromm JR, Tagliente DJ, Shaver AC, Neppalli V, Craig FE
Cytometry. Part B, Clinical cytometry 2015 Nov-Dec;88(6):413-24

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of CD32/FCGR2 was done on human peripheral blood granulocytes. Lysed whole blood was stained with a CD32/FCGR2 mouse monoclonal antibody (Product # MA1-81209) at a concentration of 0.2mg/mL for 1 hour at room temperature. Cells were washed twice with PBS, and stained with a Goat anti-Mouse IgG1 Secondary Antibody, RPE conjugate (Product # P-21129) at a dilution of 1:10 for 20 minutes at room temperature. A separate peripheral blood sample was also stained with a mouse IgG1 isotype control, RPE conjugate (Product # MG104) also at 0.2 mg/mL. Panel A shows gating of peripheral blood cell types. Panel B shows gated, stained granulocytes analyzed for CD32 fluorescent intensity. A representative 10,000 events were acquired and analyzed for each sample.