11-0329-42

antibody from Invitrogen Antibodies
Targeting: FCGR2B CD32, CD32B, FCG2, FCGR2
Provider product page for 11-0329-42

Antibody data

Product number
11-0329-42 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD32 Monoclonal Antibody (6C4 (CD32)), FITC, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: This 6C4 monoclonal antibody reacts with human CD32 (also known as Fc gammaRII). This antibody recognizes two isoforms of the receptor, Fc gammaRIIA and Fc gammaRIIB. These 40-kDa polymorphic transmembrane glycoproteins are expressed on B cells, granulocytes, monocytes, macrophages, and platelets. Moreover, these receptors are detected on natural killer cells. CD32 enables interaction between Fc gammaRII-expressing cells and opsonized antigen or IgG-containing immune complexes. Both receptors exhibit low affinity towards IgG and play a role in inflammation and autoimmune disease. This clone has been reported to inhibit Ig binding in a rosette blocking assay. Applications Reported: This 6C4 (CD32) antibody has been reported for use in flow cytometric analysis. Applications Tested: This 6C4 (CD32) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Conjugate
Green dye
Isotype
IgG
Antibody clone number
6C4 (CD32)
Vial size
100 Tests
Concentration
5 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
FCGR2B protein structure - 11-0329-42 shown in red.