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LearnPA5-47529
antibody from Invitrogen Antibodies
Targeting: HJV
haemojuvelin, hemojuvelin, HFE2, HFE2A, JH, RGMC
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- PA5-47529 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HFE2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) RGM-B is observed and less than 5% cross-reactivity with rhRGM-A is observed. Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunocytochemistry analysis of HFE2 in immersion fixed HepG2 human hepatocellular carcinoma cell line. Samples were incubated in HFE2 polyclonal antibody (Product # PA5-47529) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
