Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [2]
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- Product number
- PA5-43332 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAB5IF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: MSGGRRKEEP PQPQLANGAL KVSVWSKVLR SDAAWEDKDE FLDVIYWFRQ Sequence homology: Cow: 86%; Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 90%
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Substrate-driven assembly of a translocon for multipass membrane proteins.
Spatiotemporally-resolved mapping of RNA binding proteins via functional proximity labeling reveals a mitochondrial mRNA anchor promoting stress recovery.
Sundaram A, Yamsek M, Zhong F, Hooda Y, Hegde RS, Keenan RJ
Nature 2022 Nov;611(7934):167-172
Nature 2022 Nov;611(7934):167-172
Spatiotemporally-resolved mapping of RNA binding proteins via functional proximity labeling reveals a mitochondrial mRNA anchor promoting stress recovery.
Qin W, Myers SA, Carey DK, Carr SA, Ting AY
Nature communications 2021 Aug 17;12(1):4980
Nature communications 2021 Aug 17;12(1):4980
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human Jurkat cell lysate using an anti-C20orf24 polyclonal antibody (Product # PA5-43332).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 6 SYNJ2BP binds to specific mitochondrial mRNAs at the OMM and promotes their translation during recovery from stress. a Validation of five hits from Fig. 5d as OMM-localized RBPs by UV crosslinking-based APEX-PS. Non-RBP ACTB was used as a negative control. b Validation of RNA-binding activity using metabolic RNA labeling by 5-EU and UV crosslinking. c Confocal imaging of mitochondrial RBP orphan TAX1BP1. Anti-TOMM20 stains mitochondria and DAPI stains nuclei. Scale bars, 10 mum. d Comparison of RNA-binding activities for OMM-localized and nuclear-localized EXD2 using APEX-PS-OMM and APEX-PS-NLS. Direct streptavidin enrichment (APEX only) was performed to quantify total EXD2 protein levels in each compartment. PS only condition shows RNA-binding activity of total EXD2. e Validation of mitochondria-related SYNJ2BP clients in the absence or presence of protein translation inhibitor PUR by CLIP and qRT-PCR. IARS2 is an OMM-localized mRNA not identified to bind with SYNJ2BP. Other SYNJ2BP clients and negative controls are shown in Supplementary Fig. 10a . f Evaluation of OMM localization of SYNJ2BP clients in SYNJ2BP knockout cells by APEX-mediated proximity labeling of RNA. The OMM localization was determined by comparing APEX2-OMM with APEX2-NES labeling ( y -axis). Other SYNJ2BP clients and negative controls are shown in Supplementary Fig. 10d . g Impact of SYNJ2BP knockout on protein synthesis of SYNJ2BP-regulated clients under different cellular states. The newly synth
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 7 SYNJ2BP improves mitochondrial OXPHOS recovery and cell viability following stress. a , b Evaluation of Complex III ( a ) and IV ( b ) activities in SYNJ2BP knockout cells. c Evaluation of complex I-V assembly 12 h following treatment of HEK cells with protein translation inhibitor PUR. The quantification of each OXPHOS complex is shown below; data from three independent biological replicates. d Evaluation of cell viability 12 h following treatment of HEK cells with PUR. e , f Evaluation of protein synthesis for SYNJ2BP clients in SYNJ2BP knockout cells following heat ( e ) and sodium arsenite ( f ) stresses. The quantification of western blot data from three biological replicates is shown in Supplementary Fig. 12b-c . g Evaluation of Complex III activity in SYNJ2BP knockout cells following heat stress. h Working model of SYNJ2BP's role in retaining important (blue) mRNAs at the OMM during stress, to facilitate their rapid local translation for restoration of mitochondrial function during stress recovery (right). Two-sided Student's t -test was performed and values represent means +- SD from three biological replicates.