A03083

antibody from Boster Biological Technology

Targeting: SGO1 NY-BR-85, SGOL1

Western blot (Data presented on provider website)

Antibody Data

Product number

A03083

Provider

Boster Biological Technology

Product name

Anti-SGOL1 SGO1 Antibody

Antibody type

Polyclonal

Description

Polyclonal antibody for SGO1 detection. Host: Rabbit.Size: 100μl. Tested applications: WB. Reactive species: Human. SGO1 information: Molecular Weight: 64190 MW; Subcellular Localization: Nucleus . Chromosome, centromere . Chromosome, centromere, kinetochore . Cytoplasm, cytoskeleton, spindle pole . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Localizes to the inner centromere throughout prophase until metaphase and disappears at anaphase (PubMed:16541025). Centromeric localization requires the presence of BUB1 and the interaction with PPP2R1A (PubMed:16580887)(PubMed:16541025)(PubMed:15604152). Colocalizes with NEK2 at the kinetochore (PubMed:17621308). Colocalizes with and SS18L1 at the kinetochore (PubMed:16582621). Phosphorylation by AUKRB and the presence of BUB1 are required for localization to the kinetochore (PubMed:17617734). Isoform 1 primarily localizes to kinetochores during G2 phase and mitotic prophase, metaphase, and anaphase and does not appear to be associated with kinetochores during late mitosis (PubMed:16582621). Isoform 3 is found at the centrosome in interphase and at spindle poles in mitosis and its spindle pole localization is PLK1 dependent (PubMed:16582621). Isoform 3 does not localize to kinetochores during any stages of the cell cycle (PubMed:16582621); Tissue Specificity: Widely expressed. Highly expressed in testis. Expressed in lung, small intestine, breast, liver and placenta. Strongly overexpressed in 90% of breast cancers tested.

Reactivity

Human

Host

Rabbit

Vial size

100μl

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

Store at -20°C for one year, at 4°C for one month. We suggest the antibody be aliquoted into small vials and stored frozen at -20°C upon receiving. Avoid repeated freezing and thawing.