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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA1-10023 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Ubiquitin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Purifed from natural sources
- Description
- PA1-10023 was made against purified bovine blood derived ubiquitin coupled to keyhole limpet hemocyanin with glutaraldehyde.
- Concentration
- Conc. Not Determined
Submitted references Microglia depletion rapidly and reversibly alters amyloid pathology by modification of plaque compaction and morphologies.
The Trem2 R47H variant confers loss-of-function-like phenotypes in Alzheimer's disease.
Ubiquitin Conjugation Probed by Inflammation in Myeloid-Derived Suppressor Cell Extracellular Vesicles.
Synaptic NMDA Receptor Activation Induces Ubiquitination and Degradation of STEP(61).
Casali BT, MacPherson KP, Reed-Geaghan EG, Landreth GE
Neurobiology of disease 2020 Aug;142:104956
Neurobiology of disease 2020 Aug;142:104956
The Trem2 R47H variant confers loss-of-function-like phenotypes in Alzheimer's disease.
Cheng-Hathaway PJ, Reed-Geaghan EG, Jay TR, Casali BT, Bemiller SM, Puntambekar SS, von Saucken VE, Williams RY, Karlo JC, Moutinho M, Xu G, Ransohoff RM, Lamb BT, Landreth GE
Molecular neurodegeneration 2018 Jun 1;13(1):29
Molecular neurodegeneration 2018 Jun 1;13(1):29
Ubiquitin Conjugation Probed by Inflammation in Myeloid-Derived Suppressor Cell Extracellular Vesicles.
Adams KR, Chauhan S, Patel DB, Clements VK, Wang Y, Jay SM, Edwards NJ, Ostrand-Rosenberg S, Fenselau C
Journal of proteome research 2018 Jan 5;17(1):315-324
Journal of proteome research 2018 Jan 5;17(1):315-324
Synaptic NMDA Receptor Activation Induces Ubiquitination and Degradation of STEP(61).
Xu J, Kurup P, Nairn AC, Lombroso PJ
Molecular neurobiology 2018 Apr;55(4):3096-3111
Molecular neurobiology 2018 Apr;55(4):3096-3111
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Ubiquitin in HEK293 cell lysates. The lysate was subjected to electrophoresis on a 4-20% SDS-PAGE gel, then electrophoretically transferred to PVDF membranes. Ubiquitin on the membrane was detected using a polyclonal antibody (Product # PA1-10023) at a dilution of 1:5,000 as seen in green, and a beta-Tubulin monoclonal antibody at a dilution of 1:10,000 used as a loading control as seen in red. 1) protein standard (red), 2) cells maintained in normal medium, 3) cells treated with proteasome inhibitor lactacystin (Lc) at 10µM for 16 hours. The smear detected above the 200 kDa standard represents accumulations of ubiquitinated proteins in the Lc treated cells. The prominent band at ~8 kDa corresponds to monoubiquitin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Ubiquitin in HeLa cells. Cells were stained with a ubiquitin polyclonal antibody (Product # PA1-10023) at a dilution of 1:1,000 as seen in red, stained with a Vimentin polyclonal antibody (Product # PA1-10003) at a dilution of 1:10,000 as seen in green, and with DAPI staining the nuclear DNA as seen in blue. A) Control HeLa cells maintained in normal medium, B) HeLa cells treated with 10µM of the proteasome inhibitor lactacystin (Lc) for 24 hours. Proteasomal inhibition leads to formation of strongly ubiquitin positive cytoplasmic inclusions. Note the diffuse cytoplasmic ubiquitin staining in control cells and the well-defined ubiquitin positive inclusions in the Lc treated cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
