657403

antibody from BioLegend

Targeting: AC092143.1

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

657403

Provider

BioLegend

Proper citation

BioLegend Cat#657403, RRID:AB_2562669

Product name

Alexa Fluor® 488 anti-Tubulin Beta 3 (TUBB3)

Antibody type

Monoclonal

Antigen

Fusion protein

Description

Tubulin is the main component of microtubules. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 (TUBB3) is also found in a wide range of tumors. Studies indicate that it is a predictive and prognostic marker in various tumors.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Green dye

Antibody clone number

AA10

Vial size

25 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human lung adenocarcinoma cell line A549 was treated with BioLegend’s Fixation Buffer (Cat. No. 420801) and Permeabilization Wash Buffer (Cat. No. 421002), and then stained with TUBB3 (clone AA10) Alexa Fluor® 488 (filled hisotogram) or mouse IgG2a, κ Alexa Fluor® 488 isotype control (open histogram).

Conjugate

Green dye