MA5-31466

antibody from Invitrogen Antibodies

Targeting: TUBA1C bcm948, MGC10851, MGC14580, TUBA6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-31466

Provider

Invitrogen Antibodies

Product name

alpha Tubulin Monoclonal Antibody (GT114)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Keep as concentrated solution.

Antibody clone number

GT114

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of alpha Tubulin in various whole cell extracts using alpha Tubulin monoclonal antibody (Product # MA5-31466) using 30 µg of sample at a dilution of 1:10000. Sample was then incubated with HRP-conjugated anti-mouse IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of alpha Tubulin in whole cell extract using alpha Tubulin monoclonal antibody (Product # MA5-31466) using 30 µg of sample at a dilution of 1:10000. Sample was then incubated with HRP-conjugated anti-mouse IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of alpha Tubulin in various whole cell extracts using alpha Tubulin monoclonal antibody (Product # MA5-31466) using 30 µg of sample at a dilution of 1:10000. Sample was then incubated with HRP-conjugated anti-mouse IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of alpha Tubulin in 293T whole cell extracts using alpha Tubulin monoclonal antibody (Product # MA5-31466) at a dilution of 1:10000. Sample was then incubated with HRP-conjugated anti-mouse IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of alpha Tubulin in A) zebrafish eye lysate using alpha Tubulin monoclonal antibody (Product # MA5-31466) using 30 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-mouse IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of alpha Tubulin was performed by separating 15, 5, 2 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5-31466) at a dilution of 1:10000 and a HRP-conjugated anti-mouse IgG secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5-31466). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5-31466) diluted at 1:10,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5-31466). Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5-31466) diluted at 1:10,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5-31466). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5-31466) diluted at 1:10,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5-31466). Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5-31466) diluted at 1:10,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Tubulin alpha-1B Polyclonal Antibody detects alpha Tubulin protein by western blot analysis. Varied amount of 293T Whole cell extracts was separated by 10% SDS-PAGE, and the membrane was blotted with Tubulin alpha-1B Polyclonal Antibody alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5-31466) at a dilution of 1:10,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

alpha Tubulin Monoclonal Antibody (GT114) detects TUBA1B protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg NIH-3T3 whole cell lysate/extract. C. 30 µg mouse brain lysate/extract. D. 30 µg PC-12 whole cell lysate/extract. E. 30 µg rat brain lysate/extract. F. 30 µg drosophila lysae/extract.10% SDS-PAGE. Alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5-31466) dilution: 1:5,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

alpha Tubulin Monoclonal Antibody (GT114) detects TUBA1B protein by western blot analysis. A. 30 µg zebrafish eye lysate/extract.10% SDS-PAGE. Alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5-31466) dilution: 1:1,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TUBA1B antibody detects TUBA1B protein at Cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in -20ºC 100% methanol for 5 min. Green: TUBA1B protein stained by TUBA1B antibody (Product # MA5-31466) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

alpha Tubulin Polyclonal Antibody [GT114] detects alpha Tubulin protein expression by immunofluorescent analysis. Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Tubulin protein stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:250. Red: NeuN, stained by NeuN Polyclonal Antibody (Product # PA5-78499) diluted at 1:250. Blue: Fluoroshield with DAPI .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of alpha Tubulin was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5 31466) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

alpha Tubulin Monoclonal Antibody (GT114) detects alpha Tubulin protein at cytoplasm in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Red: alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5-31466) diluted at 1:500. Green: beta Catenin antibody [N1N2-2] (Product # PA5-77934) diluted at 1:500. Blue: Hoechst 33342 staining. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

alpha Tubulin Monoclonal Antibody (GT114) detects alpha Tubulin protein at cytoplasm in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5-31466) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

alpha Tubulin Monoclonal Antibody (GT114) detects alpha Tubulin protein at cytoplasm in human cervix by immunohistochemical analysis. Sample: Paraffin-embedded human cervix. Alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5-31466) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of alpha Tubulin in frozen mouse brain using alpha Tubulin monoclonal antibody (Product # MA5-31466) at a dilution of 1:200. Sample was then incubated with Hoechst secondary antibody.