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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [13]
- Immunocytochemistry [3]
- Immunohistochemistry [4]
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- Product number
- MA5-31466 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- alpha Tubulin Monoclonal Antibody (GT114)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (96%), Xenopus laevis (95%), Pig (100%), Rabbit (100%), Rhesus Monkey (100%), Chimpanzee (99%), Bovine (100%). Positive Control: 293T, A431, HeLa, HepG2, NIH-3T3, mouse brain, PC-12, rat brain, drosophila, zebrafish eye, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, U87-MG. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat, Drosophila, Zebrafish
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- GT114
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of alpha Tubulin in various whole cell extracts using alpha Tubulin monoclonal antibody (Product # MA5-31466) using 30 µg of sample at a dilution of 1:10000. Sample was then incubated with HRP-conjugated anti-mouse IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of alpha Tubulin in whole cell extract using alpha Tubulin monoclonal antibody (Product # MA5-31466) using 30 µg of sample at a dilution of 1:10000. Sample was then incubated with HRP-conjugated anti-mouse IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of alpha Tubulin in various whole cell extracts using alpha Tubulin monoclonal antibody (Product # MA5-31466) using 30 µg of sample at a dilution of 1:10000. Sample was then incubated with HRP-conjugated anti-mouse IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of alpha Tubulin in 293T whole cell extracts using alpha Tubulin monoclonal antibody (Product # MA5-31466) at a dilution of 1:10000. Sample was then incubated with HRP-conjugated anti-mouse IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of alpha Tubulin in A) zebrafish eye lysate using alpha Tubulin monoclonal antibody (Product # MA5-31466) using 30 µg of sample at a dilution of 1:1000. Sample was then incubated with HRP-conjugated anti-mouse IgG secondary antibody. Prior to incubation with primary antibody, the sample was separated on 10% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of alpha Tubulin was performed by separating 15, 5, 2 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5-31466) at a dilution of 1:10000 and a HRP-conjugated anti-mouse IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5-31466). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5-31466) diluted at 1:10,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5-31466). Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5-31466) diluted at 1:10,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5-31466). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5-31466) diluted at 1:10,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5-31466). Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5-31466) diluted at 1:10,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Tubulin alpha-1B Polyclonal Antibody detects alpha Tubulin protein by western blot analysis. Varied amount of 293T Whole cell extracts was separated by 10% SDS-PAGE, and the membrane was blotted with Tubulin alpha-1B Polyclonal Antibody alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5-31466) at a dilution of 1:10,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- alpha Tubulin Monoclonal Antibody (GT114) detects TUBA1B protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg NIH-3T3 whole cell lysate/extract. C. 30 µg mouse brain lysate/extract. D. 30 µg PC-12 whole cell lysate/extract. E. 30 µg rat brain lysate/extract. F. 30 µg drosophila lysae/extract.10% SDS-PAGE. Alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5-31466) dilution: 1:5,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- alpha Tubulin Monoclonal Antibody (GT114) detects TUBA1B protein by western blot analysis. A. 30 µg zebrafish eye lysate/extract.10% SDS-PAGE. Alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5-31466) dilution: 1:1,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TUBA1B antibody detects TUBA1B protein at Cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in -20ºC 100% methanol for 5 min. Green: TUBA1B protein stained by TUBA1B antibody (Product # MA5-31466) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- alpha Tubulin Polyclonal Antibody [GT114] detects alpha Tubulin protein expression by immunofluorescent analysis. Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Tubulin protein stained by alpha Tubulin Polyclonal Antibody [GT114] (Product # MA5-31466) diluted at 1:250. Red: NeuN, stained by NeuN Polyclonal Antibody (Product # PA5-78499) diluted at 1:250. Blue: Fluoroshield with DAPI .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of alpha Tubulin was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5 31466) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- alpha Tubulin Monoclonal Antibody (GT114) detects alpha Tubulin protein at cytoplasm in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Red: alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5-31466) diluted at 1:500. Green: beta Catenin antibody [N1N2-2] (Product # PA5-77934) diluted at 1:500. Blue: Hoechst 33342 staining. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- alpha Tubulin Monoclonal Antibody (GT114) detects alpha Tubulin protein at cytoplasm in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5-31466) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- alpha Tubulin Monoclonal Antibody (GT114) detects alpha Tubulin protein at cytoplasm in human cervix by immunohistochemical analysis. Sample: Paraffin-embedded human cervix. Alpha Tubulin Monoclonal Antibody (GT114) (Product # MA5-31466) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of alpha Tubulin in frozen mouse brain using alpha Tubulin monoclonal antibody (Product # MA5-31466) at a dilution of 1:200. Sample was then incubated with Hoechst secondary antibody.
