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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- 613414 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#613414, RRID:AB_2616873
- Product name
- PerCP/Cy5.5 anti-H2A.X-Phosphorylated (Ser139)
- Antibody type
- Monoclonal
- Antigen
- Modified peptide
- Reactivity
- Human
- Host
- Mouse
- Antibody clone number
- 2F3
- Vial size
- 100 tests
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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- Experimental details
- Nocodozole treated HeLa cells (24 hours) were fixed and permeabilized with cold 70% ethanol, then intracellularly stained with anti-H2A.X Phospho (Ser139) (clone 2F3) PerCP/Cy5.5 (filled histogram) or mouse IgG1, κ PerCp/Cy5.5 isotype control (open histogram).
