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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 613417 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#613417, RRID:AB_2617028
- Product name
- Direct-Blot™ HRP anti-H2A.X-Phosphorylated (Ser139)
- Antibody type
- Monoclonal
- Antigen
- Modified peptide
- Reactivity
- Human
- Host
- Mouse
- Antibody clone number
- 2F3
- Vial size
- 100 µl (20 Western blots)
- Storage
- -20°C
- Handling
- Blue Ice
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- BioLegend (provider)
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- Experimental details
- 15 µg of total protein extracts from UV untreated and treated HeLa were resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with purified or Direct-Blot™ HRP anti-H2A.X-Phospho (Ser139) antibody (clone 2F3). Proteins were visualized using chemiluminescence detection.
