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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PAB0003 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0003, RRID:AB_628568
- Product name
- H2AFX (phospho S139) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of Histone H2A.X.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Arecoline downregulates levels of p21 and p27 through the reactive oxygen species/mTOR complex 1 pathway and may contribute to oral squamous cell carcinoma.
Ji WT, Yang SR, Chen JY, Cheng YP, Lee YR, Chiang MK, Chen HR
Cancer science 2012 Jul;103(7):1221-9
Cancer science 2012 Jul;103(7):1221-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- H2AFX (phospho S139) polyclonal antibody (Cat # PAB0003). Western blot analysis of Histone-H2AX in Jurkat.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescent staining of MCF-7 that has been treated with 10 uM Camptothecin for 30 min using H2AFX (phospho S139) polyclonal antibody (Cat # PAB0003).
- Validation comment
- Immunofluorescence
