613420

antibody from BioLegend

Targeting: H2AX H2AFX

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

613420

Provider

BioLegend

Proper citation

BioLegend Cat#613420, RRID:AB_2715785

Product name

PE/Cy7 anti-H2A.X Phospho (Ser139)

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Near infrared dye

Antibody clone number

2F3

Vial size

100 tests

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Nocodazole treated HeLa cells (24 hours) were fixed and permeabilized with cold 70% ethanol, then intracellularly stained with anti-H2A.X Phospho (Ser139) (clone 2F3) PE/Cy7 (filled histogram) or mouse IgG1, κ PE/Cy7 (open histogram).

Conjugate

Near infrared dye