Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 613421 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#613421, RRID:AB_2715786
- Product name
- APC/Fire™ 750 anti-H2A.X Phospho (Ser139)
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Antibody clone number
- 2F3
- Vial size
- 25 tests
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Nocodazole treated HeLa cells (24 hours) were fixed and permeabilized with cold 70% ethanol, then intracellularly stained with anti-H2A.X Phospho (Ser139) (clone 2F3) APC/Fire™ 750 (filled histogram) or mouse IgG1, κ APC/Fire™ 750 (open histogram).