Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- MA5-24663 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Histone H2A.X Recombinant Rabbit Monoclonal Antibody (RM214)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody reacts to Histone H2AX protein, independent of post-translational modifications. No cross reactivity with other histone proteins. . Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- RM214
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot of HeLa, HEK293, A375, and SK-MEL-2 whole cell lysates, using Histone H2A.X Antibody (Product # MA5-24663) at 0.5 mg/mL, showed endogenous Histone H2AX in HeLa, HEK293, A375, and SK-MEL-2 cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells, using Histone H2A.X Antibody (Product # MA5-24663) (red). Actin filaments have been labeled with FITC-conjugated phalloidin (green).