ABIN394283

antibody from antibodies-online

Targeting: H2AX H2AFX

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

ABIN394283

Provider

antibodies-online

Product name

anti-H2A Histone Family, Member X (H2AFX) (AA 1-144) antibody

Antibody type

Monoclonal

Description

This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Reactivity

Human

Host

Mouse

Epitope

AA 1-144

Isotype

IgG

Antibody clone number

3F4

Vial size

100 μg

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles

References

Submitted references

H2AX phosphorylation screen of cells from radiosensitive cancer patients reveals a novel DNA double-strand break repair cellular phenotype.

Vasireddy RS, Sprung CN, Cempaka NL, Chao M, McKay MJ
British journal of cancer 2010 May 11;102(10):1511-8

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Phosphorylation of histone H2A.X by DNA-dependent protein kinase is not affected by core histone acetylation, but it alters nucleosome stability and histone H1 binding.

Li A, Yu Y, Lee SC, Ishibashi T, Lees-Miller SP, Ausió J
The Journal of biological chemistry 2010 Jun 4;285(23):17778-88

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Acetylation of H2AX on lysine 36 plays a key role in the DNA double-strand break repair pathway.

Jiang X, Xu Y, Price BD
FEBS letters 2010 Jul 2;584(13):2926-30

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Differences in the kinetics of gamma-H2AX fluorescence decay after exposure to low and high LET radiation.

Schmid TE, Dollinger G, Beisker W, Hable V, Greubel C, Auer S, Mittag A, Tarnok A, Friedl AA, Molls M, Röper B
International journal of radiation biology 2010 Aug;86(8):682-91

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High-resolution profiling of gammaH2AX around DNA double strand breaks in the mammalian genome.

Iacovoni JS, Caron P, Lassadi I, Nicolas E, Massip L, Trouche D, Legube G
The EMBO journal 2010 Apr 21;29(8):1446-57