- GTX127340 - GeneTex H2AX antibody

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Histone H2A.X (phospho Ser139) antibody detects H2AFX protein by western blot analysis.A. 30 ?g HCT116 whole cell lysate/extract (untreated for 2hr)B. 30 ?g HCT116 whole cell lysate/extract (UVB treatment 50mJ for 2hr)C. 30 ?g HCT116 whole cell lysate/extract (untreated for 6hr)D. 30 ?g HCT116 whole cell lysate/extract (UVB treatment 50mJ for 6hr)15% SDS-PAGEHistone H2A.X (phospho Ser139) antibody (GTX127340) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ?g of whole cell lysate) A: HCT116 control 0 hr B: HCT116 treated with 30 ?M cisplatin for 24 hr 12% SDS PAGE GTX127340 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Histone H2A.X (phospho Ser139) antibody detects H2AFX protein in cisplatin-treated samples by western blot analysis.A. 30 ?g NIH-3T3 whole cell lysate/extract (untreated) B. 30 ?g NIH-3T3 whole cell lysate/extract (30?M cisplatin treatment for 24hr)15% SDS-PAGEHistone H2A.X (phospho Ser139) antibody (GTX127340) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein by western blot analysis. Un-treated (-) and treated (+, 30 ?M cisplatin treatment for 24 hrs) PC-12 whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Untreated (¡V) and treated (+) HCT-116 whole cell extract (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Untreated (¡V) and treated (+) HCT-116 whole cell extract (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Histone H2A.X (phospho Ser139) antibody detects H2AFX protein at nuclear by confocal immunofluorescent analysis. Sample: 10uM Cisplatin treated (right) or untreated (left) HeLa cells were fixed in 4% paraformaldehyde for 15 min. Green: H2AFX protein stained by Histone H2A.X (phospho Ser139) antibody (GTX127340) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 £gm. [Images captured by Olympus FV10i Confocal Laser Scanning Microscope]

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 30 £gM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: Histone H2A.XS139ph (phospho Ser139) protein stained by Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:1000.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein at nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. Histone H2A.XS139ph (phospho Ser139) antibody (GTX127340) diluted at 1:250.

GTX127340

Antibody data