- NB100-383 - Novus Biologicals H2AX antibody

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The Journal of biological chemistry 2011 Sep 2;286(35):30806-15

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: H2AX Antibody [NB100-383] - Whole cell lysate (50 ug) from 293T cells prepared using RIPA lysis buffer. Antibody: Affinity purified rabbit anti-H2AX antibody used for WB at 0.1 ug/ml. Detection: chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: H2AX Antibody [NB100-383] - Whole cell lysate (15 ug) from NIH3T3 cells prepared using RIPA lysis buffer. Antibody: Affinity purified rabbit anti-H2AX antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Simple Western: Histone H2AX Antibody [NB100-383] - Simple Western lane view shows a specific band for H2AX in 0.2 mg/ml of HEK lysate(s). This experiment was performed under reducing conditions using the 12 - 230 kDa separation system.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Simple Western: Histone H2AX Antibody [NB100-383] - Electropherogram image(s) of corresponding Simple Western lane view. H2AX antibody was used at 10 ug/ml dilution on HEK lysate(s).

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Histone H2AX Antibody [NB100-383] - Whole cell lysate (50 ug) from HEK293T cells prepared using RIPA lysis buffer. Antibody: Affinity purified rabbit anti-H2AX antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Histone H2AX Antibody [NB100-383] - Detection of mouse H2AX by western blot. Samples: Whole cell lysate (50 ug) from F9 and L1210 cells prepared using RIPA lysis buffer. Antibody: Affinity purified rabbit anti-H2AX antibody A300-083A (lot A300-083A-5) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry: H2AX Antibody [NB100-383] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-H2AX used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: Histone H2AX Antibody [NB100-383] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-H2AX (NB100-383). Detection: DAB

NB100-383

Antibody data